HPLC Method for Separation of Sulfur-containing Biomolecules on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
These compounds are all involved in redox reactions and cellular defenses against oxidative stress in biological systems. Here’s a bit more about each of them:
Overall, these molecules play vital roles in the body’s antioxidant defenses, detoxification of xenobiotics, modulation of redox-controlled signaling pathways, and regulation of cellular proliferation and apoptosis.
These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN -10% |
| Buffer | Gradient H2SO40.1-0.3%, 10 min |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |