HPLC Method for Separation of Rutin and Kaempferol on Newcrom R1 Column
Separation type: Liquid Chromatography Reversed-phase
Rutin and kaempferol are both natural compounds found in various plants, particularly in fruits and vegetables. They belong to the class of flavonoids, which are a group of polyphenolic compounds known for their antioxidant properties and potential health benefits.
Both rutin and kaempferol are considered bioactive compounds, and their presence in a balanced diet rich in fruits and vegetables is associated with potential health benefits.
Rutin and kaempferolcan be retained, separated and analyzed on a reversed-phase Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 265 nm using UV detection
Methodenparameter
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 10-90%,10 min , 5 min hold |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 265 nm |
| Sample | 1. 0.5 mg/ml 2. 0.5 mg/ml |
| Injection Volume | 5.0 µL |