HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. Both are naturally occurring compounds found in Amanita muscaria and related species of mushrooms. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |