HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode
Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.
Methodenparameter
| Column | Primesep 200 , 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210, 245, 315 nm |