HPLC Method for Separation BHA and BHT on Newcrom R1 Column
Separation type: Liquid Chromatography Reversed-phase
3-tert-butyl-4-hydroxyanisole (BHA) is a synthetic antioxidant commonly used as a food preservative. It is an aromatic compound with the chemical formula C11H16O2. BHA belongs to a class of compounds known as butylated hydroxyanisoles.
Function: BHA is used as an antioxidant to prevent the spoilage of fats and oils in various food products. It helps protect these substances from oxidative degradation, extending the shelf life of processed foods.
Chemical Structure: The compound has a molecular structure with a tert-butyl group (tert-butyl radical) attached to the oxygen atom of a hydroxyanisole group. The IUPAC name for BHA is 2-tert-butyl-4-methoxyphenol.
Usage: BHA is commonly used in a variety of food products, including snack foods, baked goods, cereals, and fats and oils. It is also used in certain cosmetic and pharmaceutical products.
Butylated Hydroxytoluene (BHT) is a synthetic antioxidant belonging to the class of compounds known as butylated hydroxytoluenes. Like BHA (butylated hydroxyanisole), BHT is commonly used as a food preservative to prevent the oxidation of fats and oils in various processed foods. It is also used in other products, including cosmetics, pharmaceuticals, and industrial applications.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 50 -90%, 6 min, 4 min hold |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 280 nm |
| Sample | 1 mg/ml |
| Injection Volume | 1 µL |