HPLC Method for Analysis of Xanthurenic acid on Newcrom BH Column
Separation type: Liquid Chromatography Mixed-mode
Xanthurenic acid is a compound associated with tryptophan metabolism and is known to be excreted in the urine. It is a non-essential, non-proteinogenic amino acid derivative and is considered to be a metabolite of tryptophan. Here are some key points about xanthurenic acid:
Xanthurenic acid is characterized by the presence of two hydroxy groups and a carboxylic acid group. Structurally, it is composed of a bicyclic ring system that incorporates a benzene ring and a pyridine ring (with a nitrogen atom). The benzene ring is substituted with two hydroxy groups at positions 4 and 8, and the pyridine ring carries a carboxylic acid functionality.
Xanthurenic acid can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 200 nm
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 10-60%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | 250 nm |