HPLC Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.
You can find detailed UV spectra of Vitamin B1 (Thiamine) , Vitamin B2 (Riboflavin) , Nicotinamide , Vitamin B6 (Pyridoxine) , Pyridoxamine , Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins ( B1 , B2 , B3 , B6 )
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | Gradient H2SO4 -0.1-0.5% in 10 min |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm |