Separation of Vitamins in Mixed-Mode HPLC
Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.
Methodenparameter
| Column | Primesep D , 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |