HPLC Method for Analysis of Thiamine on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Thiamine, also known as vitamin B1, is an essential nutrient that plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells.
Vitamin B1 (Thiamine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.
You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 -0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |
| Injection Volume | 1 µl |