HPLC Method for Analysis of Sucralose on Primesep S2 Column

HPLC-Applikation • Datum: 2025/08/12
HPLC Method for Analysis of Sucralose on Primesep S2 Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose

Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8 .

Properties: Appearance: Typically a white, odorless, crystalline powder.

Molecular weight: ~397.6 g/mol

Solubility: Soluble in water.

Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.

Methodenparameter

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN – 95%
BufferFormic Acid – 0.1%
Flow Rate1.0 mL/min
DetectionELSD
Limit of Detection3.152 ppm