HPLC Method for Analysis of Quinolinic acid on Newcrom BH Column
Separation type: Liquid Chromatography Mixed-mode
Quinolinic acid (also known as quinolinate or 2,3-pyridinedicarboxylic acid) is a dicarboxylic acid with a pyridine backbone. It plays a role in various biological processes and is associated with some pathological conditions.
Quinolinic acid can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and posphoric acid. This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 200 nm
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | 200 nm |