HPLC Method for Analysis of Prednisone on Primesep B Column
Prednisone is a synthetic corticosteroid drug that belongs to the class of glucocorticoids. It is a prodrug, meaning that it is biologically inactive until it undergoes metabolic activation in the liver, where it is converted into its active form, prednisolone. Both prednisone and prednisolone have anti-inflammatory and immunosuppressant properties and are used for various medical conditions.
Prednisone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 35% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 245 nm |