HPLC Method for Analysis of Mellitic Acid on Newcrom BH Column

HPLC-Applikation • Datum: 2024/03/01
HPLC Method for Analysis of Mellitic Acid on Newcrom BH Column Chromatogramm

Separation type: Liquid Chromatography Reversed-phase

Mellitic Acid is an aromatic dicarboxylic acid with the chemical formula C12H6O12. It is a white crystalline solid and is derived from benzoic acid. Mellitic acid is named after mellite, a mineral in which it was first discovered. It is not commonly encountered in everyday use and does not have widespread applications.

Chemical Structure: Mellitic acid consists of a benzene ring with six carboxylic acid functional groups (-COOH) attached to it, forming a hexacarboxylic acid.

Occurrence: Mellitic acid is not a naturally abundant compound and is primarily known for its existence as a mineral, mellite, which contains mellitic acid.

Uses: Due to its structure and properties, mellitic acid does not have significant practical applications in industry or daily life. It is more commonly encountered in scientific contexts and research.

Derivatives: Mellitic acid can form various derivatives, and some of its salts and esters have been studied for specific purposes.

Methodenparameter

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN/H2O
BufferH2SO4 – 0.1%
Flow Rate1.0 mL/min
DetectionUV 220 nm
Sample0.25 mg/ml
Injection Volume1 µL