HPLC Method for Analysis of Lactulose on Primesep S2 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Lactulose
Lactulose is an synthetic disaccharide with the molecular formula C12H22O11 .
Properties: Appearance: Typically a white, odorless crystalline powder with a sweet taste.
Molecular weight: ~342.30 g/mol
Solubility: Soluble in water.
Uses: Used for treating constipation and hepatic encephalopathy (HE).
Methodenparameter
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 80% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD |
| Limit of Detection | 7.643 ppm |