HPLC Method for Analysis of Kynurenic Acid on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Kynurenic acid (KYNA) is a metabolite in the kynurenine pathway, which degrades the amino acid tryptophan. It acts as an antagonist to receptors in the brain, such as NMDA and alpha-7 nicotinic acetylcholine receptors, playing a dual role in the central nervous system. KYNA is neuroprotective by inhibiting excitatory neurotransmission and reducing oxidative stress, but elevated levels are linked to cognitive impairments and disorders like schizophrenia and depression.
Kynurenic Acid can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 240 nm.
You can find detailed UV spectra of Kynurenic Acid and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN from 5% to 50% |
| Buffer | Gradient H2SO4from 0.1% to 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 240 nm |