HPLC Method for Analysis of Isophthalic acid on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Isophthalic acid is an aromatic dicarboxylic acid with the chemical formula C6H4(CO2H)2. It is a colorless crystalline solid and is one of the three isomers of benzene dicarboxylic acid, the others being phthalic acid and terephthalic acid. In isophthalic acid, the two carboxyl groups (−COOH) are positioned on the 1st and 3rd carbons of the benzene ring, making it a meta isomer.
Isophthalic acid can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 210 nm.
You can find detailed UV spectra of Isophthalic acid and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 30-70%, 10 min |
| Buffer | H3PO4 – 0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210 nm |
| Limit of Detection | 5 ppb |