HPLC Method for Analysis of Hydrocortisone on Newcrom CN2 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydrocortisone
Hydrocortisone is an organic compound with the molecular formula C21H30O5 .
Properties: Appearance: Typically a white to practically white, odorless, crystalline powder.
Molecular weight: ~362.5 g/mol
Solubility: Soluble in organic solvents like ethanol.
Uses: A corticosteroid medication used to treat various conditions by reducing inflammation and suppressing the immune system.
Methodenparameter
| Column | Newcrom CN2, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 240 nm |