HPLC Method For Analysis of Ethylene Glycol and Polyethylene Glycol (PEG) on Primesep AP Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG200, PEG400.
Polyethylene glycol ( PEG ) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals.
HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.
Methodenparameter
| Column | Primesep AP, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 95/5% |
| Buffer | No |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm, RI |