HPLC Method for Analysis of Dicarbon Carboxylic Acids on Newcrom BH Column
Separation type: Liquid Chromatography Mixed-mode
These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 Å, dual ended column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | H2O -99.7% |
| Buffer | HClO4 – 0.3 |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |