HPLC Method for Analysis of D-alpha-Tocopheryl acetate
High Performance Liquid Chromatography (HPLC) Method for Analysis of D-alpha-Tocopheryl acetate
D-alpha-Tocopheryl acetate is a form of Vitamin E with a molucular formula of C31H52O3 . It is also known as alpha-tocopheryl acetate. It is often used in cosmetics, food, and dietary supplements due to its antioxidant properties.
D-alpha-Tocopheryl acetate can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 4.6 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 98/2% |
| Buffer | H3PO4 – 0.02% |
| Flow Rate | 2.0 mL/min |
| Detection | UV, 270 nm |