HPLC Method for Analysis of Citrulline on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Citrulline .
Citrulline is an alpha-amino acid with the chemical formula C6H13N3O3 . It is a key intermediate in the urea cycle. It can be derived from a variety of sources, such as arginine, ornithine, and asymmetric dimethylarginine. Commercially, it is used as a dietary supplemant as L-citrulline boots the production of nitric oxide, which helps with arterial function.
Citrulline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | H2SO4 – 0.1% |
| Buffer | MeCN/H2O – 10/90% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |