HPLC Analysis of Citrulline and Arginine on Primesep Columns
Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.
Methodenparameter
| Column | Primesep, 2.1×50 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 0.2 mL/min |
| Detection | ELSD |