HPLC Method for Analysis of Carbamazepine on Primesep 100 Column

HPLC-Applikation • Datum: 2025/07/17
HPLC Method for Analysis of Carbamazepine on Primesep 100 Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbamazepine

Carbamazepine is an organic compound with the molecular formula C15H12N2O .

Properties: Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Methodenparameter

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN – 35%
BufferH2SO4 – 0.2%
Flow Rate1.0 mL/min
DetectionUV 212 nm
Limit of Detection5 ppb