HPLC Method for Analysis of Butylated Hydroxyanisole (BHA) on Newcrom R1 Column on Cromite™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Butylated Hydroxyanisole (BHA)
Butylated Hydroxyanisole ( BHA ) is a petrochemical with the chemical formula C11H16O2 . Due to it’s antioxidant properties, it is used in preservation of food, cosmetics, and pharmaceuticals. Due to previous experiments in animals, it is suspected to be a carcinogen for humans.
Butylated Hydroxyanisole (BHA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |