HPLC Method for Analysis of Brilliant Green on Primesep 100 Column
Brilliant green , also known as zelyonka and zelenka , is a triarylmethane dye with the chemical formula C27H33N2 .HO4S . A dilute alcoholic solution of Brilliant Green is used as a topical antiseptic. At lower solutions, it can also be used for mucous membranes and for infants. It is a visible light-activated photocatalyst in organic synthesis
Brilliant Green can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 600 nm |