HPLC Method for Analysis of Berberine on Primesep B Column
Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).
Biological Activities:
Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 266 nm
Methodenparameter
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 5-20%, 10 min |
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 mL/min |
| Detection | λ max 230, 266, 348, 431 nm |
| Injection Volume | 1 µl |