HPLC Method for Analysis of Ascorbic Acid on Newcrom BH Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbic Acid
Ascorbic Acid is a vitamin with the molecular formula C6H8O6 . Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus.
Ascorbic Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 |