HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol
4-Amino-2-nitrophenol is an organic compound with the molecular formula C6H6N2O3 .
Properties: Appearance: Typically a yellow to orange crystalline solid
Molecular weight: ~154.12 g/mol
Solubility: Soluble in water and polar organic solvents
Uses: Intermediate in the manufacture of dyes, pigments, and pharmaceuticals
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Limit of Detection | 93 ppb |