HPLC-ELSD Method for Analysis of Amino Acids on Primesep 100 Column
11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).
Methodenparameter
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | TFA , gradient 0.05-0.3% , 25 min |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD |