HPLC Dye Separation on SHARC1 Column
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column . Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Methodenparameter
| Column | Sharc 1, 3.2×100 mm, 3 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/EtOH – 99/1% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.5 mL/min |
| Detection | UV, 500, 650 nm |