HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine (FD&C Yellow 5) .
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430 nm.
You can find detailed UV spectra of Yellow 5 and information about its various lambda maxima by visiting the following link.
Methodenparameter
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 430 nm |