HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Primesep AB Column
Epinephrine and epinephrine sulfonate were analyzed using Primesep AB, a reverse-phase column with both acidic and basic embedded ion-pairing groups. This method is LC/MS compatible.
Methodenparameter
| Column | Primesep AB, 4.6×250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.25% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 280 nm |