HILIC Separation of Surfactant Triton X-100 on Obelisc N Column

HPLC-Applikation • Datum: 2008/08/22
HILIC Separation of Surfactant Triton X-100 on Obelisc N Column Chromatogramm

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)

Methodenparameter

ColumnObelisc N, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferAmAc pH 5.0
Flow Rate1.0 mL/min
DetectionUV 250, 230 nm