Carbofuran Separation on Obelisc R HPLC Column

HPLC-Applikation • Datum: 2015/08/24
Carbofuran Separation on Obelisc R HPLC Column Chromatogramm

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Methodenparameter

ColumnObelisc R, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN – 20%
BufferAmFm pH 3.0- 20 mM
Flow Rate1.0 mL/min
DetectionUV, 250 nm