Application USP Methods for the Analysis of Atenolol using the Legacy L1 Column
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Application USP Methods for the Analysis of Atenolol using the Legacy L1 Column
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Atenolol .
Atenolol is a prescription beta-blocker with the chemical formula C 14 H 22 N 2 O 3 . It is used to treat hypertension and high blood pressure. It can also improve survival chances after a heart attack. Off-label, it is also used to treat migraines and prevent anxiety.
Atenolol can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 85-60%, 10 min 2 min hold |
|---|---|
| Buffer | Gradient AmFm pH 3.0, 10-80 mM, 10 min 2 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | beta-blocker |
| Analyzing Compounds | Atenolol |
HPLC Column Used
Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended