Application USP Methods for Nitrofurantoin for the Legacy L1
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Application USP Methods for Nitrofurantoin for the Legacy L1
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitrofurantoin .
Nitrofurantoin is an oral antibiotic with the chemical formula C 8 H 6 N 4 O 5 . It is used to treat urinary tract infections.
Nitrofurantoin can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 43/55% |
|---|---|
| Buffer | NaHPO4 pH3.5 – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 233 nm |
| Class of Compounds | Drug, Acid, Ionizable, Hydrophobic |
| Analyzing Compounds | Nitrofurantoin |
HPLC Column Used
Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended