USP Methods Analysis of Procainamide on Legacy L1 Column

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Chromatogram

Description

· Application Columns: Legacy L1 C18 HPLC column · Application compounds: Procainamide and 4-aminobenzoic acid · Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid · Detection technique: UV · Reference: USP35: NF30

Method Parameters

Mobile PhaseWater/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
BufferTEAPh
Flow Rate1.5 ml/min
DetectionUV, 230 nm
Class of CompoundsDrug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing CompoundsIbuprofen, benzophenone

HPLC Column Used

Legacy L1, 4.6×150 mm, 5 µm, 100A
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