HPLC Method for Substituted Furans Separation on Newcrom R1 column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of 5-Hydroxymethyl-2-furaldehyde (5-HMF) , Furfuryl alcohol , Furfural , 2-Furancarboxylic acid , 3,4-Bis(hydroxymethyl)furan , 5-Hydroxymethyl-2-furancarboxylic acid .

Furan is a chemical contaminant that forms in some foods during traditional heat treatment techniques, such as cooking, jarring, and canning.

5-Hydroxymethyl-2-furaldehyd e ( 5-HMF ), also known as Hydroxymethylfurfural , is an organic compound with the chemical formula C 6 H 6 O 3 . It can form in sugar-containing food when heated. Due to being considered a potential carcinogen, much research has gone into it’s formation.

Furfuryl alcohol is a trapping agent with the chemical formula C 5 H 6 O 2 . It is used to synthesize ethyl levulinate, prepare collodial microporous carbon spheres, and as a starting material to prepare cyclopentanone.

Furfural is an organic compound with the chemical formula C 5 H 4 O 2 . IT is found primarily in cooked or heated coffee and whole grain bread. It is used as a renewable chemical feedstock, which can be converted into a variety of useful chemicals.

2-Furancarboxylic acid is an organic compound with the chemical formula C 5 H 4 O 3 . It is used primarily in the production of furoate esters, some of which are drugs and pesticides.  As a flavoring, it is used for it’s sweet, oily, and earthly flavor.

5-Hydroxymethyl-2-furaldehyde (5-HMF) , Furfuryl alcohol , Furfural , 2-Furancarboxylic acid , 3,4-Bis(hydroxymethyl)furan , 5-Hydroxymethyl-2-furancarboxylic acid can be retained and separated in HPLC using Newcrom R1 reverse-phase column.  The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 210 and 275 nm.

Method Parameters

HPLC Column Used