HPLC Method for the Simultaneous Separation of Melamine and Cyanuric Acid on an Obelisc N Column
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Description
Melamine is an organic basic compound that is derived from cyanamide. It contains 66% nitrogen by mass. Addition of melamine is used to increase protein count in milk products. Cyanuric acid is a trimer of cyanic acid. Melamine and cyanuric acid can form a insoluble complex. Both compounds has been linked as additives to modify nitrogen count in products. Cyanuric acid is a slightly acidic hydrophilic compound, while melamine is a basic hydrophilic compound. Both compounds were separated on Obelisc R reversed-phase ion-exchange column with LC/Ms compatible mobile phase. Method can be used for generic analysis of basic and acidic compounds with LC/MS compatible conditions.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Acid, Hydrophobic, Ionizable |
| Analyzing Compounds | Melamine, Cyanuric Acid |
HPLC Column Used
Obelisc N, 4.6 x 150 mm, 5 µm, 100 A, dual ended