Separation of Methotrexate on Newcrom R1 HPLC column

CALCULATED CHROMATOGRAM
https://sielc.com/separation-of-methotrexate-on-newcrom-c18-hplc-column
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Chromatogram

CALCULATED CHROMATOGRAM – NOT AN EXPERIMENTAL RESULT
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data.

Description

Methotrexate is a drug used in chemotherapy and in suppressing the immune system. It is used to treat a lot of cancers and autoimmune diseases. Methotrexate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics. .

Calculated chromatograms: Where marked, the chromatogram was calculated by a proprietary SIELC algorithm and may deviate from the actual experimental data.

Data source & disclaimer: The HPLC application data, chromatograms and method parameters on this page are sourced from SIELC Technologies and are provided for informational and educational purposes only. All trademarks, column names and product designations remain the property of their respective owners. AugustaChrom does not claim ownership of any SIELC application data.
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