HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column
https://sielc.com/application-separation-of-metal-ions-on-primesep-100-columnChromatogram
Description
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30% |
|---|---|
| Buffer | TFA- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Magnesium, Calcium, Lithium, Barium |
HPLC Column Used
Primesep 100, 4.6×250 mm, 5 µm, 100A