Separation of 2,3,5,6-Tetrachlorohydroquinone on Newcrom R1 HPLC column

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Chromatogram

Description

2,3,5,6-Tetrachlorohydroquinone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.