LC MS-HPLC Method for Analysis of Defactinib on Primesep B Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Defactinib
Defactinib is a focal adhesion kinase (FAK) inhibitor with the chemical formula C 20 H 21 F 3 N 8 O 3 S . It was developed by Pfizer as potential treatment for solid tumors. You can find detailed UV spectra of Defactinib and information about its various lambda maxima by visiting the following link.
Defactinib can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium format as a buffer. Detection is performed using ESI+Sim and UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60 % |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ESI+SIM 511+, ([M+H]1⁺) UV 277, 305 nm |
| LOD* | ESI-SIM 4 ppb |
| Class of Compounds | Inhibitor |
| Analyzing Compounds | Defactinib |
HPLC Column Used
Primesep B, 2.1 x 100 mm, 5 µm, 100 A, dual ended