LC MS-HPLC Method for Analysis of Aceclidine on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Aceclidine · Aceclidine Drug class: Muscarinic cholinergic agonist
Mechanism of action: Stimulates muscarinic receptors, leading to smooth muscle contraction and glandular secretion.
Historical use: Studied for glaucoma, gastrointestinal motility, and other parasympathetic-related effects
2025 FDA Approval:
You can find detailed UV spectra of Aceclidine and information about its various lambda maxima by visiting the following link.
Aceclidine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium format as a buffer. Detection is performed using ESI+ SIM.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60 % |
|---|---|
| Buffer | AmFm pH 3.0 – 60 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ESI+SIM 170+, ([M+H]1⁺) |
| Class of Compounds | Drug |
| Analyzing Compounds | Aceclidine |