HPLC Method For Analysis Of Histidine and Histidine Methyl Ester Dihydrochloride on Primesep 100 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Histidine hydrochloride monohydrate , Histidine , Histidine methyl ester .
L-Histidine is an essential amino acid with the chemical formula C 9 H 11 N 3 O 2 . It cannot be produced within the body and must be obtained through consuming it. It s fundamental for repair of damaged tissue, growth of muscles, and making of blood cells. Outside of protein, it also has the unique property of being able to act as a buffer to help maintain stable pH levels in the body. Sources of it include meat, fish, dairy products, beans, and nuts. You can find detailed UV spectra of Histidine and information about its various lambda maxima by visiting the following link.
Histidine Methyl Ester , also written is HME , is an irreversible histidine decarboxylase inhibitor with the chemical formula C 7 H 11 N 3 O 2 .
L-Histidine hydrochloride monohydrate , Histidine , Histidine methyl ester can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amino acid |
| Analyzing Compounds | L-Histidine hydrochloride monohydrate,Histidine,Histidine methyl ester |