HPLC Method for Separation of Naphthalene and Sulfonated Naphthalene Compounds on Chromni Column

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Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,5-Naphthalenedisulfonic Acid , 2-Naphthalenesulfonic Acid , Naphthalene .

Naphthalene is an aromatic hydrocarbon with the chemical formula C 10 H 8 . It is primarily used as a precursor to derivative chemicals. The largest use of it is in the industrial production of phthalic anhydride. You can find detailed UV spectra of 1,5-Naphthalenedisulfonic Acid , 2-Naphthalenesulfonic Acid , Naphthalene and information about its various lambda maxima by visiting the following link.

2-Naphthalenesulfonic Acid is an organic compound with the formula C 10 H 7 SO 3 H . It is used primarily in the production of dyes, but has also historically been industrially used for a it’s various chemical reactions.

Armstrong’s acid , also known as is naphthalene-1,5-disulfonic acid , is an acid with the chemical formula C 10 H 6 (SO 3 H) 2 . It is occasionally used as a divalent counterion for forming salts as an alternative to mesylate and tosylate salts. You can find detailed UV spectra of 1,5-Naphthalenedisulfonic Acid , 2-Naphthalenesulfonic Acid , Naphthalene and information about its various lambda maxima by visiting the following link.

1,5-Naphthalenedisulfonic Acid , 2-Naphthalenesulfonic Acid , Naphthalene can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Method Parameters

Mobile PhaseMeCN/H2O
BufferAmmonium Acetate pH 4.0 – 10 mM
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsNaphthalene
Analyzing Compounds1,5-Naphthalenedisulfonic Acid,2-Naphthalenesulfonic Acid,Naphthalene

HPLC Column Used

Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended