HPLC Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom BH
https://sielc.com/hplc-uv-determination-of-sodium-3-chloro-2-hydroxypropane-1-sulfonate-2Chromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate .
Sodium 3-chloro-2-hydroxypropane-1-sulfonate is an anionic agent with the chemical formula C 3 H 6 ClNaO 4 S . It is used to prepare negatively charged probe particles to investigate the nanoporosity of cellulosic fibers and their interactions with cationic polyelectrolytes.
Sodium 3-chloro-2-hydroxypropane-1-sulfonate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeOH/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Sulfonate |
| Analyzing Compounds | Sodium 3-chloro-2-hydroxypropane-1-sulfonate |
HPLC Column Used
Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended