HPLC UV Method for Analysis of Oligonucleotide 5A on Chromni Column
https://sielc.com/hplc-uv-analysis-of-oligonucleotide-5aChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides
Oligonucleotide 5A is a small RNA molecule with a specific adenine sequence. They are primarily used to inhibit viral and cancer cell growth. In RNA synthesis, they are intermediates in enzymatic synthesis of capped RNAs.
Oligonucleotides can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium acetate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 75 – 55%, 10 min |
|---|---|
| Buffer | Ammonium Acetate pH 5.0 – 10mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 |
| Class of Compounds | Oligonucleotide |
| Analyzing Compounds | Oligonucleotides |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated