HPLC UV Method for Analysis of Oligonucleotide 12-mer on Chromni Column
https://sielc.com/hplc-uv-analysis-of-oligonucleotide-12-merChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides · Research and Studies : · Medical and Diagnostic Applications : · Educational Purposes : · Demonstrating basic principles of nucleic acid chemistry and genetics. · Nanotechnology :
In DNA nanotechnology, specific sequences of DNA are used to form structures and shapes at the nanoscale. A poly(A) sequence might be part of a larger structure.
Method Parameters
| Mobile Phase | Gradient MeCN – 75 – 60%, 20 min |
|---|---|
| Buffer | Ammonium Acetate pH 5.0 – 10mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 |
| Class of Compounds | Oligonucleotide |
| Analyzing Compounds | Oligonucleotides |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated