HPLC UV Method for Analysis of Oligonucleotide 10A on Chromni Column
https://sielc.com/hplc-uv-analysis-of-oligonucleotide-10aChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides
Oligonucleotide 10A is a modified oligonucleotide used in screening gene libraries as well as research using PCR, DNA sequencing, labeled probes, plasmid construction, and genomic manipulations
Oligonucleotides can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 75 – 55%, 15 min |
|---|---|
| Buffer | Ammonium Acetate pH 5.0 – 5mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 |
| Class of Compounds | Oligonucleotide |
| Analyzing Compounds | Oligonucleotides |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated